2186 ) 0. Ans. (b) SDS–PAGE
Ref: Harper’s Biochemistry, pg. 24
• The most widely used method for determining the purity of
a protein is SDS-PAGE—polyacrylamide gel electrophoresis
(PAGE) in the presence of the anionic detergent sodium
dodecyl sulfate (SDS)
• Electrophoresis separates charged biomolecules based
on the rates at which they migrate in an applied electrical
field. For SDS-PAGE, acrylamide is polymerized and
crosslinked to form a porous matrix. SDS denatures and
binds to proteins at a ratio of one molecule of SDS per
two peptide bonds.


.


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2186 ) 0. Ans. (b) SDS–PAGE
Ref: Harper’s Biochemistry, pg. 24
• The most widely used method for determining the purity of
a protein is SDS-PAGE—polyacrylamide gel electrophoresis
(PAGE) in the presence of the anionic detergent sodium
dodecyl sulfate (SDS)
• Electrophoresis separates charged biomolecules based
on the rates at which they migrate in an applied electrical
field. For SDS-PAGE, acrylamide is polymerized and
crosslinked to form a porous matrix. SDS denatures and
binds to proteins at a ratio of one molecule of SDS per
two peptide bonds.


.


📍 Practice more questions daily at @PrepNEXTHUB and attend daily tests at @PrepNEXT at 9:30 pm

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304 viewsJun 26 at 09:11