Lab_science | United States America (US)

Create: 2025-02-12 Update: 2025-07-07 21:53:04

در ادامه روش برای انجام تست CAMP را بررسی خواهیم کرد.
روش استاندارد
ابتدا یک خط مستقیم از استافیلوکوکوس اورئوس تولید کننده بتا همولیزین در مرکز پلیت حاوی آگار خون ایجاد کنید.
سپس، یک رگه از ارگانیسم مورد آزمایش (استرپتوکوک بتا همولیتیک که باید شناسایی شود) عمود بر رگه استافیلوکوک تلقیح کنید. مراقب باشید که خط استافیلوکوک را قطع نکنید.
نکته: این رگه‌ها را به گونه‌ای بسازید که پس از انکوباسیون، رشد دو جاندار با هم تماس نداشته باشد.
طول رگه استرپتوکوک باید 3 تا 4 سانتی‌متر باشد.
به طور مشابه، سویه‌های استرپتوکوک شناخته شده گروه A و B را در همان پلیت با کنترل منفی و مثبت تلقیح کنید.
از برچسب زدن محل هر رگه در پشت صفحه اطمینان حاصل کنید.
در نهایت پلیت را در دمای 35 درجه سانتی گراد به مدت 18 الی 24 ساعت انکوبه کنید.

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در ادامه روش برای انجام تست CAMP را بررسی خواهیم کرد.

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