Endpoint and Rate (kinetic) Reactions:
==============================
🔰When an analyte is detected using a chemical reaction, there are two options for measuring its concentration.
✝️One is to wait until the reaction is complete and the total amount of analyte is converted to product – called an endpoint reaction.

✝️The other is to measure the rate of change in product formed over time – called a rate reaction.

Endpoint Reactions.
🔰Endpoint reactions are especially suitable for chemical reactions which are completed in a relatively short time and produce one product molecule or complex for each molecule of analyte.
👉For example, a reaction of albumin with the dye bromocresol purple (BCP) produces a colored complex. If the reaction is allowed to continue until all the albumin present in solution has reacted and the maximum amount of colored product has formed, the color at the end of the reaction reflects the total amount of albumin as the albumin-dye complex.

🔰Endpoint reactions can measure the creation of a product or the loss of reactant.
🔰If the method measures the creation of a product, the absorbance is higher at the endpoint than at the start point (called an end-up reaction).
🔰If the method measures the disappearance of a reactant, the absorbance is lower at the endpoint than at the start point (called an end-
down reaction).

https://hottg.com/+AAAAAECuqoTkgQkHpiHlGA

Clinical 🅱iochemistry

Endpoint and Rate (kinetic) Reactions:
==============================
🔰When an analyte is detected using a chemical reaction, there are two options for measuring its concentration.
✝️One is to wait until the reaction is complete and the total amount of analyte is converted to product – called an endpoint reaction.

✝️The other is to measure the rate of change in product formed over time – called a rate reaction.

Endpoint Reactions.
🔰Endpoint reactions are especially suitable for chemical reactions which are completed in a relatively short time and produce one product molecule or complex for each molecule of analyte.
👉For example, a reaction of albumin with the dye bromocresol purple (BCP) produces a colored complex. If the reaction is allowed to continue until all the albumin present in solution has reacted and the maximum amount of colored product has formed, the color at the end of the reaction reflects the total amount of albumin as the albumin-dye complex.

🔰Endpoint reactions can measure the creation of a product or the loss of reactant.
🔰If the method measures the creation of a product, the absorbance is higher at the endpoint than at the start point (called an end-up reaction).
🔰If the method measures the disappearance of a reactant, the absorbance is lower at the endpoint than at the start point (called an end-
down reaction).

https://hottg.com/+AAAAAECuqoTkgQkHpiHlGA

hottg.com/Biochem_Lab/732

10.3K viewsOct 6, 2017 at 15:31